Part:BBa_K112000:Design

T4 holin, complete CDS, berkeley standard

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

Source

PCR from existing part.